The TSC Biosample Repository houses human biological materials such as blood, DNA, and tissues linked to detailed clinical data in the TSC Natural History Database. High-quality biosamples and their associated clinical data will enable researchers to discover biomarkers, establish human cell lines or tissue arrays for drug testing, and search for clues to understand why TSC is so different from person to person.
Types of samples available include:
- DNA isolated from white blood cells and buccal cells
- White blood cell pellets
- Remnant tissue from surgeries (frozen or fixed, paraffin-embedded), including brain, kidney, and liver
In the near future, the TSC Biosample Repository will provide researchers in North and South America access to the TSC1- and TSC2-knockout HEK293T cell lines from the Nellist laboratory at Erasmus MC after quality control analysis of the cell lines is complete:
- Cell line 1C2 (TSC1-/-)
- Cell line 3H9 (TSC2-/-)
- Cell line 3H9-1B1 (TSC1-/-/TSC2-/-)
- HEK 293T (parental cell line)
The Van Andel Research Institute in Grand Rapids, MI processes, stores, and delivers TSC Biosample Repository samples on behalf of the TS Alliance.
Participant Characteristics as of March 2018 (n=291)
Please see this report for more information about the representativeness of the participants in the Biosample Repository.
How to Request Samples
All biosample requests will be reviewed by the Biosample Use Committee:
- Peter Crino, MD, PhD (University of Maryland)
- David J. Kwiatkowski, MD, PhD (Brigham and Women’s Hospital)
- Jeff Mackeigan, PhD (Michigan State University)
- Debora Moritz (TSC community member)
- Steve Roach, MD (University of Texas at Austin)
- Steven L. Roberds, PhD (Tuberous Sclerosis Alliance)
- Dana Valley (Van Andel Research Institute)
The TS Alliance is eager to partner with sponsors of clinical trials or clinical research studies to collect biosamples centrally at the TSC Biosample Repository. Such biosamples will remain under the control of the study’s biosample use committee until the conclusion of the project, at which time the samples will become part of the openly available TSC Biosample Repository. This provides a win-win opportunity to ensure the long-term availability of valuable samples. This process is being used with the Developmental Synaptopathies Consortium and the PREVeNT clinical trial.
Information for Individuals with TSC and Their Families
If you are an individual with TSC or a family member of someone with TSC, please see here for information about donating samples to the TSC Biosample Repository.
The TSC Biosample Repository is governed and wholly funded by the TS Alliance thanks to generous support from Bill and Kristina Watts, the Cowlin Family Fund, the Engles Collaborative Research Fund, Jim and Andrea Maginn, and many additional donors through the Unlock the Cure campaign.